Till date, whole genome gene expression analysis by applying microarray technology, has been the dominant technique in Toxicogenomics. Over recent years, more ‘omics platforms have been put to use for investigating molecular networks of toxicity, proteomics and metabolomics . Where such a combination of technologies seems of added value, insights in the increasing complexity of gene regulation networks have forced the Toxicogenomics research community has also absorbed even more upfront techniques, such as microRNA analysis and whole genome DNA methylation analysis.
The advent of latest generation separation (UPLC) and analytical techniques (high resolution, high speed, high accuracy mass spectrometers) enable the quantitative analysis of biological events at the proteome level.
With respect to mitochondrial functioning, Electron Spin Resonance spectroscopy is already available for directly identifying and quantifying radical formation, and the most effective and direct method for detecting free radicals
LuxCel has developed a series of oxygen-sensitive fluorescent probes for the assessment of both isolated mitochondria and whole cells. LuxCel has also developed a series of probes for assessing intracellular oxygen. The xCELLigence system for measuring heart electrophysiology in vitro is available.